Even one drop of liquid from raw poultry or meat can contain enough Campylobacter to make a person ill.2 Using the right testing methods in your facility is important to help mitigate safety risks for food products.
The U.S. Department of Agriculture Food Safety and Inspection Service (USDA FSIS) has recently chosen a reliable technology for detecting Campylobacter in poultry products by selecting the 3M™ Molecular Detection Assay 2-Campylobacter as their labs’ primary method for detection of this pathogen.1
For poultry processors, a solution for testing two types of bacteria simultaneously is available: the 3M™ Molecular Detection System with 3M™ Molecular Detection Assay 2 – Campylobacter and 3M™ Molecular Detection Assay 2 – Salmonella. With a single protocol for both pathogens post-enrichment, processors are able to perform up to 96 tests of multiple types in one 60-minute run. In addition to the assay, 3M offers Campylobacter Enrichment Broth, a simplified enrichment that requires only sterile water, eliminating one of the many steps in traditional monitoring methods.
In 2018, the USDA chose the 3M™ Molecular Detection System as a primary method for Salmonella3 and Listeria monocytogenes4 testing for meat, poultry and egg products. It is anticipated the USDA FSIS’ recent designation of MDS as its chosen method for Campylobacter testing will be added to the USDA Microbiology Laboratory Guidebook (MLG) updates and published later this year.
The advantages of LAMP technology
The 3M™ Molecular Detection System is a fast, accurate, easy-to-use and affordable solution powered by unique DNA-based technology — Loop-Mediated Isothermal Amplification (LAMP).
Gabriela Lopez Velasco, Ph.D., is a senior global technical service expert in 3M’s Food Safety Lab. She explains the advantages of using LAMP rather than polymerase chain reaction (PCR), which has been used for pathogen detection for more than 30 years: “PCR testing requires numerous cycles of heating and cooling to amplify the target – and that calls for more complex equipment.”
LAMP uses isothermal amplification, meaning it only needs to be heated up to one temperature – 60 to 65 degrees Celsius. That means fewer steps for the technician and smaller, simpler equipment. The 3M Molecular Detection System also uses bioluminescence to detect the amplification reaction, allowing detection of the target pathogen in as little as 15 minutes.
Gaby sees advantages for food safety technicians. “With PCR, there are multiple steps,” she says. “For lysis of enriched samples, you add the enzyme, heat it and cool it before transfer to amplification reaction leading to multiple transfer steps,” she says. “With LAMP there are only two transfers and amplification at only one temperature.”
Interested in learning more about the 3M Molecular Detection System? Talk to a 3M food safety expert
- United States Department of Agriculture Food Safety and Inspection Service (2021). FSIS Constituent Update – April 2, 2021, Vol. 24, No. 31. Retrieved from ConstUpdate_04.02.2021_.pdf (usda.gov).
- New York State Department of Health Campylobacteriosis Fact Sheet (ny.gov)
- Isolation and identification of salmonella from meat, poultry, pasteurized egg, and siluriformes (fish) products and carcass and environmental sponges. USDA. January 2019. Available at: https://www.fsis.usda.gov/sites/default/files/media_file/2021-03/mlg-4.pdf
- Isolation and identification of listeria monocytogenes from red meat, poultry, ready-to-eat siluriformes (fish) and egg products, and environmental samples. USDA. January 2019. Available at: https://www.fsis.usda.gov/sites/default/files/media_file/2021-03/mlg-8.pdf